Table 1.
List of strains, recorded data on imipenem susceptibilities, PAP profiles, imipenemase production and molecular data.
| B. fragilis | Ref. | Phenotypic Parameters a | Molecular Parameters | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| cfiA and IS | qRT-PCR b | Lrp c | |||||||||||||||||||
| x0 PBS | x0 BHIS | bS PBS | bS HIS | d PBS | d BHIS | AUC PBS | AUC BHIS | MIC B | MIC WC | IP HRI | Ipase | cfiA | cfiA-IS | cfiA | ‘GNAT’ | ‘XAT’ | ‘GNAT/XAT’ | K no. | K% | ||
| Susceptible controls | |||||||||||||||||||||
| NCTC 9343 | - | −4.8142 | −4.3392 | −0.2092 | −0.3902 | 2 | 2 | 1 | 1 | 0.125 | 0.064 | 0 | 0 | - | n.a. | 0 | 0 | 0 | n.a. | n.a. | n.a. |
| 638R | - | −4.4449 | −4.9148 | −0.2721 | −1.0111 | 2 | 2 | 1.4757 | 1.4046 | 0.125 | 0.064 | 0 | 0 | - | n.a. | 0 | 0 | 0 | n.a. | n.a. | n.a. |
| D39 | [22] | −4.4350 | −5.7965 | −0.1012 | −0.2192 | 2 | 2 | 1.4886 | 0.7642 | 0.5 | 0.064 | 0 | 0 | - | n.a. | 0 | 0 | 0 | n.a. | n.a. | n.a. |
| Silent/HR d | |||||||||||||||||||||
| 7979 | This study | −3.0103 | −3.5941 | −0.4000 | −0.8885 | 3 | 4 | 1.8045 | 1.5884 | 0.125 | 0.032 | 0 | 5.1 | + | - | 16.35 | 6.91 | 5.56 | 1.2428 | 7 | 26.9 |
| 3130 | This study | −2.7685 | −3.0168 | −0.6269 | −0.0451 | 3 | 6 | 1.8785 | 1.5532 | 8 | 8 | 0 | 2.6 | + | - | 1 | 1 | 1 | 1 | 5 | 13.5 |
| 3035 | [7] | −2.8450 | −2.7613 | −0.3141 | −0.3887 | 3 | 3 | 1.7810 | 1.4387 | 1 | 2 | 5 | 9.7 | + | - | 166.3 | 0.02 | 0.993 | 0.0201 | 9 | 25.0 |
| SY69 | [22] | −2.2774 | −3.4300 | −0.2062 | −1.5269 | 5 | 6 | 2.0114 | 1.7008 | 4 | 4 | 5 | 10.1 | + | - | 92.0 | 17.7 | 32.6 | 0.5429 | 5 | 13.5 |
| CZE65 | [10] | −2.6235 | 0.9301 | −0.9546 | −1.1148 | 5 | 5 | 2.6027 | 2.3992 | 16 | 8 | 2 | 25.5 | + | - | 2.46 | 1.93 | 0.16 | 12.0625 | 7 | 26.9 |
| CZE60 | [10] | 0.0886 | −0.7637 | −1.4586 | −0.6320 | 3 | 4 | 2.7013 | 1.8329 | 16 | 16 | 5 | 33.3 | + | - | 12.91 | 7.78 | 0.49 | 15.8775 | 7 | 26.9 |
| SLO8 | [9] | 1.8171 | −4.0275 | −0.6387 | −1.1367 | 3 | 8 | 2.7294 | 2.2562 | 128 | 128 | 3 | 133 | + | - | 76.06 | 182.94 | 255.47 | 0.7161 | 8 | 25.0 |
| HR-ind d | |||||||||||||||||||||
| 3130i5 | This study | −0.3129 | −2.1373 | −1.2807 | −0.5161 | 4 | 8 | 2.9720 | 2.4650 | 4 | 4 | 5 | 28 | + | - | 5.91 | 52.18 | 18.67 | 2.80 | 5 | 13.5 |
| CZE60i2 | This study | 3.1618 | −2.7092 | −0.4220 | −2.0045 | 3 | 5 | 3.0608 | 2.4110 | 128 | 64 | 6 | 36.8 | + | - | 22.93 | 8.78 | 0.33 | 26.6061 | 7 | 26.9 |
| With IS d | |||||||||||||||||||||
| De248514/19 | This study | 0.2977 | −0.5716 | −3.9655 | 3 | 4 | 2.4364 | 2.1085 | 16 | 64 | 3 | 134 | + | IS614B | 509.8 | 460.1 | 3.7 | 124.3514 | 8 | 25.0 | |
| 1672 | [8] | 2.1815 | −7.1023 | −0.8173 | 2 | 3 | 2.6174 | 2.5047 | 256 | 256 | 4 | 189 | + | IS1168 | 2.02 | 10.49 | 185.59 | 0.0565 | 7 | 26.9 | |
| TAL3636 | [23] | 3.5263 | 3.3190 | −0.6621 | −0.4304 | 2 | 4 | 2.8433 | 2.7366 | 128 | 256 | 0 | 354 | + | IS942 | 97.5 | 0.024 | 0.697 | 0.0343 | 8 | 25.0 |
a Abbreviations as follows: bS PBS—b parameter from PBS suspensions; bS BHIS—b parameter from BHIS broth cultures; d PBS—fold/dilution increase in PBS PAPs; d BHIS—fold/dilution increase in BHIS PAPs; Ipase—specific imipenemase production (u/mg cell extract). b Q-RT-PCR expressions and the relative expression (ratio of expressions) of ‘GNAT-XAT’. c K no.—number of lysines in Lrp; K %—the lysine content in Lrp in percentages. d Types of cfiA-positive strains: silent without IS elements but showing some HR, induced HR and strains with IS activated cfiA genes.