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. 2022 Apr 30;11(5):691. doi: 10.3390/biology11050691

Table 1.

Microsatellite primers used for molecular characterization of Ariza labeo (L. ariza).

Locus Primer Sequence Annealing Temperature (°C)
Lr1 F-GAAAGCTGCTCGTCCTTGAA
R-GAAAGCTGCTCGTCCTTGAA
53
Lr2 F-GGGTGTGGGAGAGAAAGAGAG
R-GGAGTCTGACAAATGCAGCAAG
62
Lr3 F-ATCTGGCTGCCTAATCACC
R-CATCGGCGACTGCACTGGA
58
Lr22 F-GATCTGTGTGTGTGTGTGC
R-GGTGGCGACACAACAAATG
58
Lr24 F-CAAGGCGAAAAGTGTCCAT
R-AAGAAATTGGTAAAGTGTTTC
56
Lr27 F-TGGAAATCGAAGGCGTTTCCAC
R-AGCACTTACAGTCCATTGGCTC
60
Lr28 F-AAAGGAAACAGACTCCATCAG
R-CGCTAGCACTTTAATTTCACAGAG
50
Lr29 F-CCCACGCAAACTCCTGTT
R-GGAACAAGGCCAGAGCTTTA
50