TABLE 3.
Growth kinetics for bacterial isolates grown to steady state in nitrogen-limited chemostat cultures
| Taxon | Temp (°C) | Steady-state NO3− concn (μM) | μmax with NO3− (h−1) | Knit (μM) | anito (liter · μmol−1 · h−1) | Steady-state NH4+ concn (μM) | μmax with NH4+ (h−1) | Kamm (μM) | aammo (liter · μmol−1 · h−1) |
|---|---|---|---|---|---|---|---|---|---|
| Vibrio logei | 15 | 2.5 (0.1)a | 0.0677 (0.0003) | 6.9 | 0.0098 | 9.3 (0.1) | 0.0614 (0.002) | 22.4 | 0.0027 |
| 10 | 8.5 (0.1) | 0.053 (0.001) | 16.5 | 0.0032 | 10.8 (0.5) | 0.053 (0.003) | 20.8 | 0.0025 | |
| 8 | 8.5 (0.3) | 0.0375 (0.005) | 9.2 | 0.0041 | 9.1 (0.01) | 0.048 (0.001) | 15.1 | 0.0032 | |
| 6 | 11.0 (0.1) | 0.0373 (0.0001) | 11.8 | 0.0032 | 4.3 (0.2) | 0.049 (0.005) | 7.4 | 0.0066 | |
| 4 | 13.5 (0.1) | 0.0381 (0.001) | 15.1 | 0.0025 | 11.4 (0.2) | 0.0391 (0.002) | 13.4 | 0.0029 | |
| Hydrogenophaga pseudoflava | 15 | NGb | NG | NG | NG | 1.6 (0.02) | 0.074 (0.001) | 5.0 | 0.0148 |
| 10 | NG | NG | NG | NG | 4.5 (0.4) | 0.06 (0.009) | 10.5 | 0.0057 | |
| 5 | NG | NG | NG | NG | 1.6 (0.03) | 0.06 (0.002) | 3.7 | 0.016 | |
| Brevibacterium sp. | 15 | NG | NG | NG | NG | 2.7 (0.7) | 0.12 (0.02) | 13.2 | 0.0091 |
| 10 | NG | NG | NG | NG | 4.5 (0.04) | 0.08 (0.01) | 15.5 | 0.0052 | |
| 5 | NG | NG | NG | NG | 6.4 (0.1) | 0.055 (0.004) | 15.4 | 0.0036 | |
| Klebsiella oxytoca | 20 | 2.5 (0.1) | 0.143 (0.005) | 17.3 | 0.0083 | 2.7 (0.02) | 0.128 (0.008) | 16.5 | 0.0078 |
| 15 | 5.0 (0.1) | 0.116 (0.007) | 27.2 | 0.0043 | 2.8 (0.03) | 0.105 (0.002) | 13.6 | 0.0077 | |
| 10 | 9.5 (0.5) | 0.087 (0.01) | 36.6 | 0.0024 | 3.1 (0.03) | 0.092 (0.003) | 12.8 | 0.0072 | |
| Escherichia coli | 35 | 11 (0.05) | 0.212 (0.004) | 118.6 | 0.0018 | 8.7 (0.1) | 0.172 (0.0001) | 74.4 | 0.0023 |
| 25 | 12 (1) | 0.15 (0.009) | 88.0 | 0.0017 | 6.2 (0.04) | 0.064 (0.001) | 15.8 | 0.0041 | |
| 20 | 14.5 (0.1) | 0.17 (0.011) | 122.4 | 0.0014 | NMc | NM | NM | NM | |
| 15 | 26 (0.3) | 0.08 (0.0001) | 89.6 | 0.0009 | 8.1 (0.2) | 0.037 (0.001) | 8.6 | 0.0043 | |
| Bacillus stearothermophilus | 45 | 5.5 (0.1) | 0.144 (0.007) | 38.4 | 0.0038 | 5.5 (0.1) | 0.144 (0.01) | 38.4 | 0.0038 |
| 40 | 6.0 (0.2) | 0.098 (0.007) | 26.6 | 0.0037 | 7.1 (0.06) | 0.096 (0.005) | 30.7 | 0.0031 | |
| 35 | 8.5 (0.02) | 0.071 (0.012) | 24.9 | 0.0029 | 3.4 (0.04) | 0.078 (0.007) | 11.3 | 0.0069 | |
| 30 | 13.5 (0.1) | 0.058 (0.003) | 29.9 | 0.0019 | 6.3 (0.3) | 0.067 (0.0001) | 17.0 | 0.0039 | |
| 25 | NG | NG | NG | NG | 5.3 (0.05) | 0.058 (0.005) | 11.61 | 0.0050 |
a
The values in parentheses are standard errors of the means (n = 3).
b
NG, no growth.
c
NM, not measured.