Figure 2.

Selenite treatment improves insulin sensitivity under lipotoxic conditions. (a) Representative protein phosphorylation, expression, and densitometric analysis of members of the insulin signaling cascade in mature 3T3-L1 adipocytes treated with or without selenite during differentiation and after 100 nM insulin stimulation. (b) Representative protein phosphorylation, expression, and densitometric analysis of members of the insulin signaling cascade in 3T3-L1 preadipocytes pre-treated with or without palmitate for three days, with or without selenite for further three days, and after 100 nM insulin stimulation. (c) Oil-Red-O absorption and representative staining of 3T3-L1 adipocytes differentiated in the absence or presence of selenite and palmitate for 8 days. *: p < 0.05, ***: p < 0.001, ****: p < 0.0001 after two-tailed Student’s t-test. All data are presented as mean ± SEM.