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. 2022 May 9;11(5):928. doi: 10.3390/antiox11050928

Figure 3.

Figure 3

Effect of SOD3 on the secretion of matrix metalloproteinase (MMP)-1 and type I collagen in fibroblasts. Human foreskin fibroblasts were incubated in a serum-free medium in the presence of TNF-α (1 ng/mL) and/or SOD3 (3 μg/mL) for 24 h. The secreted levels of MMP-1 and type I collagen in the conditioned media were assessed via western blot analysis using antibodies against MMP-1 and pN-COL1A1, respectively. GAPDH levels in cell lysates were analyzed as a loading control. Graphs show the levels of MMP-1 and type I collagen relative to the levels in non-treated cells, which were quantified using the ImageJ software. Each value represents the mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control. n.s.: not significant.