Skip to main content
. 2022 May 10;11(5):940. doi: 10.3390/antiox11050940

Figure 2.

Figure 2

Confocal imaging of proteins modified by 2-ClHDyA and 2-ClHyA in CHO.K1 (A) and FAA.K1A (B) cells. CHO.K1 and FAA.K1A treated with 2-ClHDyA and 2-ClHyA as described in Figure 1 were subjected to confocal microscopy as described in Section 2. TAMRA in red indicates localization of proteins modified by 2-ClHDyA and 2-ClHyA and blue is DAPI nucleus staining; (C) Total corrected cell fluorescence was measured using ImageJ from CHO.K1 and FAA.K1A cells treated with DMSO, 10 µM 2-ClHDyA or 10 µM ClHyA following 1 h incubation. Mean + SEM **** p < 0.0001 and not significance (ns) for comparison within 2-ClHDyA and 2-ClHyA treatment between CHO.K1 and FAA.K1A cells, respectively.