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2-ClHDyA modified EA.hy926 cell proteins. Silver-stained gel (A) and Western blot (B) probed with streptavidin HRP of the samples from A were prepared as described in Section 2. Protein loads showing total proteins from lysates from each indicated condition (DMSO vehicle, HDyA (non-chlorinated aldehyde control) and 2-ClHDyA) input onto beads, elute from beads, and supernatant (Flow Through); (C) Venn diagram of number of 2-ClHDyA- and HDyA-modified proteins in EA.hy926 cells.
