Figure 1.

Effect of the cryopreservation process on the (a) viability, (b) mobility, and (c) osmotic tolerance of turkey sperm. Mean values ± SE (n = 5) of sperm qualitative parameters recorded for either fresh or frozen–thawed turkey sperm were reported. (a) Viability values, expressed as %, were measured by means of a dual staining technique (as shown in inset) using the stains SYBR-14 (green, viable cells) and PI (red, dead cells). (b) Mobility values, expressed as Abs 550 nm, were measured by the sperm motility test. (c) Sperm osmotic tolerance (SOT) was assessed by the hypo-osmotic H₂O test. For further details, see Methods section. * = p < 0.05.