Skip to main content
. 2022 Apr 26;11(5):661. doi: 10.3390/biology11050661

Figure 3.

Figure 3

Venom serine protease-like zymography. (A) 50 µg of venom protein in a 10% SDS-PAGE gel. (B) Trypsin-like activity was determined using BApNA as the substrate and incubated for 2.5 h. (C) 100 µg of venom protein in a 10% SDS-PAGE gel. (D) Chymotrypsin-like proteolytic activity determined using SAAFpNA as the substrate and incubated for 2.5 h. Hybrids of the first hybridization event (11 years old): MH1, FH3, and MH4; hybrids of the second hybridization event (10 years old): MH6 and MH7; pooled venom of four male organisms of C. aquilus (MCa-PV), pooled venom of four female organisms of C. polystictus (FCp-PV). MWM, molecular weight marker.