Figure 8.
Protein fractionation of the venom by RP-HPLC. Pooled venoms of C. aquilus (A), C. polystictus (B), the male hybrids (MH1, MH4, MH6, and MH7) (MHs) (C), and a female hybrid (FH3) (D). Red asterisks show the peaks not shared between the samples. SVSP, snake venom serine protease; SVMPs, snake venom metalloprotease; SVPLA2, snake venom phospholipase; SVH, snake venom hyaluronidase. Absorbance units (AUs) at 280 nm. Enzymatic activity was not determined. The family proposed for the enzymes was based on similarity of the elution profiles with the reported data.