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. 2022 Apr 23;11(5):646. doi: 10.3390/biology11050646

Figure 3.

Figure 3

Oxidative stress and apoptosis. (A) Oxyblot in cardiac samples and the graph shows the measurements of optical density expressed as arbitrary optical density unit (ODU). Cardiac lysates were immunoblotted with anti-4-Hydroxynonenal (4-HNE) (B) and anti-Caspase-3 (C). Graphs show the densitometric ratios of bands and β-actin expression, used to normalize the data. CHOW rats (n = 8), reference group fed with standard diet; DIO rats (n = 9), fed with high-fat diet; DS (n = 12), DIO rats supplemented with tart cherry seeds; DJS (n = 9), DS rats supplemented with tart cherry juice. Data are mean ± SEM. * p < 0.05 vs. CHOW rats; # p < 0.05 vs. DIO rats.