Figure 1.

Flow cytometric detection of CB₁ and CB₂ in and on RASF. RASF were incubated with TNF (10 ng/mL) for 72 h, and CB₁ and CB₂ levels were determined thereafter. Upper panel: histogram; detection of CB₁ at the plasma membrane (PM) and intracellularly and CB₂ at the plasma membrane. Lower panel: violin plots; quantification of CB₁ and CB₂. t–test was used for comparisons. p = 0.05 was the level of significance.