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. 2022 May 19;10(5):1171. doi: 10.3390/biomedicines10051171

Figure 6.

Figure 6

Plaques in the proximal aorta from ApoE−/− Gsdmd−/− mice show decreased macrophage infiltration as compared to ApoE−/− Gsdmd+/+ controls. ApoE−/− Gsdmd−/− and ApoE−/− Gsdmd+/+ mice were fed a WD for 16 weeks. Sections of the proximal aorta were stained with (A) hematoxylin/eosin to quantify plaque size, necrotic cores (# hash signs), and cell infiltration; (B) Sirius red to measure total collagen content; (C) anti-MAC3 and anti-α-smooth muscle actin (αSMA) to determine macrophage and smooth muscle cell content, respectively, and to calculate the ratio of αSMA/MAC3 immunoreactivity; (D) TUNEL to count apoptotic cells (dotted boxes are magnified, scale bar = 20 μm). * p < 0.05, ** p < 0.01 (independent samples t-test, boxplot: Mann–Whitney test, n = 13–18 mice per group). Scale bar = 100 μm. Representative images are shown.