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. 2022 May 14;12(5):701. doi: 10.3390/biom12050701

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Transmission electron microscope section of a hepatocyte obtained from disaggregation by Medimachine II. (B) Transmission electron microscope section of a hepatocyte obtained from enzymatic dissociation. (C) Confocal section of liver cells obtained from disaggregation with Medimachine II, in green MTG-labeled cytoplasm and in red PI-labeled nuclei. Scale bar: 10 μm. (D) Transmission electron microscope section of a lymphocyte obtained from enzymatic procedure. (A,B,D). Scale bars: 2 μm. (E) Flow cytometry histograms of cell cycle analyses of both procedures.