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. 2022 May 3;10(5):1056. doi: 10.3390/biomedicines10051056

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of hypoxia on the mRNA profile expression of DPSCs. (A) Real-time RT-qPCR analysis of the levels of expression of early and late markers of neural differentiation (nestin, GFAP, EGF, NGF, bFGF BDNF, GDNF) after 5 days and after 16 days of hypoxic exposure. (B) Secreted growth factors bFGF and EGF were detected in the conditioned media of DPSCs exposed or not exposed to hypoxia for 5 days and 16 days by human EGF and FGF basic ELISA Kits. Histograms indicate the means ± SE of three different cultures, each of which was tested in triplicate. Statistical analysis was performed by Student’s t-test; * p < 0.05; ** p < 0.01 vs. 5N.