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. 2022 Apr 29;10(5):1032. doi: 10.3390/biomedicines10051032

Figure 3.

Figure 3

Effect of EMPA on Hippo signalling pathway in CDAHFD-induced mice. (a) Western blot of mice liver protein extracts with the indicated antibodies. (b) Quantitation and normalisation of phospho-specific signal to total protein or total protein to β-actin from a. (c) The relative expression of cyr61, cTGF, and AREG was determined via real-time PCR. ** p < 0.01, and *** p < 0.001 in the comparisons of the CD-fed mice with the CDAHFD-fed mice. † p < 0.05, †† p < 0.01, and ††† p < 0.001 in the comparisons of the CDAHFD-fed mice with the EMPA treatment groups.