Figure 1.

OsOSCA1.1 mediates hyperosmolality-induced [Ca²⁺]_cyt (OICI_cyt) and salt-induced [Ca²⁺]_cyt (SICI_cyt) increases in rice seedling roots. (A–C) OICI_cyt was monitored in the root cells of ZH11 and ososca1.1, expressing Yellow Cameleon YC3.6 (YC3.6) under treatment with 250 mM of sorbitol. E_app, relative apparent Förster resonance energy transfer (FRET) efficiency of YC3.6. (A) Typical image before and after treatment, (B) changes in [Ca²⁺]_cyt over time, and (C) mean change in [Ca²⁺]_cyt in response to 250 mM of sorbitol in ZH11 and ososca1.1 [means ± standard deviation (SD); n = 40]. In (B), black and red lines indicate [Ca²⁺]_cyt in ZH11 and ososca1.1, respectively; black arrow indicates hyperosmolality stress initiation. In (C), black dots indicate individual experiments; black and red lines indicate mean changes. (D–F) SICI_cyt was analyzed in the root cells of ZH11 and ososca1.1 expressing YC3.6 under treatment with 100 mM of NaCl. (D) Typical image before and after treatment, (E) changes in [Ca²⁺]_cyt over time, and (F) mean change in [Ca²⁺]_cyt in response to 100 mM NaCl in ZH11 and ososca1.1 (means ± SD; n = 40). In (E), black and red lines indicate [Ca²⁺]_cyt in ZH11 and ososca1.1, respectively; black arrow indicates salt stress initiation. In (F), black dots indicate individual experiments; black and red lines indicate mean changes.