Figure 3.

Types of interneurons generated from iPSCs. Immunofluorescence analysis of idNs stained with antibodies against interneuronal subtypes markers (A) somatostatin (SST), (B) calretinin (CALB2), and (C) calbindin (CALB1). In each group of images, WT cells are shown in the upper panel, while SCN1A^M145T idNs are shown in the lower panel. White arrows in the merged images indicate neurons expressing the interneuronal makers indicated (63× magnification). (D–F) Quantification of percentage of MAP2⁺ neurons co-expressing interneuronal markers immunostained in panels (A–C). About 9–1% of idNs express SST and CALB2, while CALB1 is present in less than 1% percent of neurons. At least 200 cells were counted for each bar, and data are presented as mean ± SEM of two independent experiments.