Figure 5.

Expression of chloride co-transporters in idNs. (A,B) qRT-PCR analysis of chloride co-transporters KCC2 and NKCC1 in neurons from WT and SCN1A^M145T tested at day of differentiation 0 (NSCs), d14, d21, d28 and d60. (C) NKCC1/KCC2 mRNA ratio in idNs at different days of differentiation. The ratio was calculated as the inverse of ΔCt_NKCC1/ΔCt_KCC2: (ΔCt = Ct_{Gene_Of_Interest} − Ct_GAPDH). For all graphs, the mean ± SEM of three biological replicates is shown; ** p < 0.01, *** p < 0.001, t-test has been calculated vs. WT at the same day of differentiation.