Table 2.
Phenolic compounds tentatively identified in the hydroethanolic extracts of Cynara cardunculus L. var altilis blades collected at different growth stages. The retention time (Rt), wavelength of maximum absorption (λmax) in the UV-Vis region, and mass spectral data are presented.
| Peak | Rt (min) | λmax (nm) | [M-H]− (m/z) | MS2 (m/z) | Tentative Identification |
|---|---|---|---|---|---|
| 1 | 4.50 | 327 | 353 | 191 (100), 179 (5), 161 (5), 135 (5) | 3-O-Caffeoylquinic acid |
| 2 | 6.69 | 266 | 153 | 109(100) | Protocatechuic acid |
| 3 | 6.80 | 326 | 353 | 173 (100), 179 (11), 191 (10), 161 (5), 135 (5) | 4-O-Caffeoylquinic acid |
| 4 | 7.01 | 326 | 353 | 191 (100), 179 (10), 161 (5), 135 (5) | cis 5-O-Caffeoylquinic acid |
| 5 | 7.11 | 326 | 353 | 191 (100), 179 (7), 173 (5), 135 (5) | trans 5-O-Caffeoylquinic acid |
| 6 | 10.43 | 326 | 353 | 191 (100), 179 (11), 161 (5), 135 (5) | Caffeoylquinic acid derivate |
| 7 | 16.13 | 285/sh324 | 463 | 287 (100) | Eriodictyol-O-hexuronoside |
| 8 | 16.83 | 322 | 515 | 353 (100), 335 (26), 191 (64), 17 9(10) | cis 1,3-Di-O-caffeoylquinic acid |
| 9 | 18.49 | 345 | 461 | 285 (100) | Luteolin-O-hexuronoside derivative I |
| 10 | 18.70 | 334 | 515 | 353 (100), 179 (35), 173 (29), 353 (10), 191 (10), 135 (8), 161 (5) | trans 1,3-Di-O-caffeyolquinic acid |
| 11 | 18.78 | 345 | 461 | 285 (100) | Luteolin-O-hexuronoside derivative II |
| 12 | 18.93 | 340 | 447 | 285 (100) | Luteolin-O-hexoside |
| 13 | 20.41 | 344 | 515 | 353 (100), 191 (12), 335 (10) | cis 1,5-Di-O-cafffeoylquinic acid |
| 14 | 20.50 | 328 | 515 | 353 (100), 191 (5), 335 (12) | trans 1,5-Di-O-cafffeoylquinic acid |
| 15 | 22.66 | 329 | 515 | 353 (100), 335 (5), 229 (3), 255 (5), 203 (6), 191 (69), 179 (12), 173 (4, MS3 base peak) | cis 3,4-Di-O-cafffeoylquinic acid |
| 16 | 22.70 | 329 | 515 | 353 (100), 335 (5), 229 (3), 255 (6), 203 (3), 191 (76), 179 (11), 173 (5, MS3 base peak) | trans 3,4-Di-O-cafffeoylquinic acid |
| 17 | 22.89 | 329 | 515 | 353 (100), 335 (32), 191 (20), 179 (12) | cis 3,5-Di-O-cafffeoylquinic acid |
| 18 | 23.65 | 332 | 533 | 489 (100), 285 (20) | Luteolin-O-malonyl hexoside derivative I |
| 19 | 23.67 | 346 | 533 | 489 (50), 447 (5), 285 (100) | Luteolin-O-malonyl hexoside derivative II |
| 20 | 25.60 | 330 | 515 | 353 (100), 191 (13, MS3 base peak) | trans 3,5-Di-O-caffeolyquinic acid |