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The percent viability of frozen–thawed sexed semen by PY-microbeads. Con: conventional frozen–thawed semen; NC: conventional semen incubated with nonactive PLA-M microbeads; X-enriched: sperm unbound by PY-microbeads; Y-enriched: sperm entrapped by PY-microbeads; A, B, C = comparison of dead sperm in each group; a, b, c = comparison of live sperm in each group.
