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. 2022 May 6;11(5):715. doi: 10.3390/biology11050715

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Discrimination between X- and Y-sperm after sexing by PY magnetic beads. (a) Regions are utilized to analyze sperm bearing the X and Y chromosomes. Separate populations of sperm bearing X and Y chromosomes were isolated, and the sex ratio in each sample was evaluated. (b) Patterns of sperm were observed with Hoechst 33,342. (c) Frequency histogram and percentage of sperm after staining with Hoechst 33,342. Con: conventional frozen–thawed semen; NC: conventional semen incubated with inactive magnetic PLA-M; X-enriched: fraction of sperm not bound by PY-magnetic beads; Y-enriched: fraction of sperm entrapped by PY-magnetic beads. * Paired t-tests were used to compare X- and Y-sperm from each group. p-value < 0.05 is statistically significant.