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Expression levels of PLP and SRY genes in X- and Y-enriched semen samples were measured by RT–PCR to validate the sexing technique for bull semen. Con: conventional frozen–thawed semen; X-enriched: fraction of sperm not bound by PY-magnetic beads; Y-enriched: fraction of sperm entrapped by PY-magnetic beads; Sort X: commercial cell-sorter-sexed semen; A, B = comparison of PLP amplicons from each group; a, b = comparison of SRY amplicons from each group.
