Table 4.
Acrosome integrity of frozen–thawed sexed semen by PY-microbeads (n = 20; mean ± SD).
| Parameter | Treatment | p-Value | |||
|---|---|---|---|---|---|
| T1 | T2 | T3 | |||
| CON | NC | (X-Enriched) | (Y-Enriched) | ||
| LI | 42.40 ± 7.25 b | 40.20 ± 6.25 b | 38.90 ± 7.77 b | 12.44 ± 3.25 a | 0.001 |
| DI | 30.77 ± 4.42 a | 35.72 ± 5.52 a | 40.20 ± 7.25 a | 60.72 ± 9.01 b | 0.004 |
| LR | 0.77 ± 0.04 b | 0.82 ± 0.24 b | 0.44 ± 0.05 a | 0.42 ± 0.12 a | 0.032 |
| DR | 26.06 ± 2.94 | 23.26 ± 4.35 | 20.46 ± 8.88 | 26.42 ± 1.24 | 0.125 |
Con: conventional frozen–thaw semen; NC: conventional semen incubated with non-active PLA-M microbeads; X-enriched: unbound sperm with PY-microbeads; Y-enriched: entrapped sperm with PY- microbeads; LI: live-acrosome-intact sperm; LR: live acrosome-reacted sperm; DI: dead acrosome-intact sperm; DR: dead acrosome-reacted sperm. Different superscript letters in the same row indicate significant differences (p < 0.05).