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. 1999 Jun;65(6):2622–2630. doi: 10.1128/aem.65.6.2622-2630.1999

FIG. 2.

FIG. 2

A proposed enzyme-linked assay for 232-MB. In this assay the 232-MB would be isomerized to 321-MB by a putative isomerase. 321-MB would subsequently be oxidized to 3-methyl-2-buten-1-al by a 321-MB dehydrogenase with the concomitant reduction of NAD+ to NADH. The production of NADH would then be monitored by photometric or fluorometric means.