TABLE 1.

Induction of 321-MB and benzyl alcohol dehydrogenase activities in P. putida MB-1

Carbon source	Cell growth (A600)	Activitya of:
		321-MB dehydrogenase (nmol min−1A600−1)	Benzyl alcohol dehydrogenase (nmol min−1A600−1)
Hcy	1.58	<0.5b	<0.5b
Hcy + 5 mM 232-MB	2.46	61	58
Hcy + 50 mM 232-MB	4.41	85	93
Hcy + 5 mM 321-MB	2.39	10	9
Hcy + 5 mM 2-methyl-2-butanol	1.44	<0.5	<0.5
Hcy + 23 mM l-leucine	6.58	<0.5	<0.5
Hcy + 5 mM benzyl alcohol	1.58	<0.5	<0.5
Hcy + 50 mM glucose	8.49	<0.5	<0.5
Hcy + 50 mM ethanol	4.66	<0.5	<0.5
Hcy + 5 mM 1-butanol	2.41	13	16

^a321-MB and benzyl alcohol dehydrogenase activities were measured with permeabilized cells grown on Hcy medium with various carbon sources as described in Materials and Methods. Cell growth was assessed by the A₆₀₀ of duplicate cultures, and dehydrogenase activities in permeabilized cells were measured at least twice and normalized for cell density based on the A₆₀₀ of the reaction mixtures; the averages of these values are presented in the table. Data are results of a typical experiment repeated two times. 

^bThe detection limit in these assays was about 0.5 nmol min⁻¹ A₆₀₀⁻¹.