TABLE 3.
Kinetic parameters for 321-MB dehydrogenase with various substratesa
| Substrate | Structure | Apparent Vmax (U mg−1) | Apparent Km (μM) | Specificity, Vmax/Km (U mg−1 μM−1) |
|---|---|---|---|---|
| Allylic and nonallylic 3-Chloro-2-buten-1-ol |  |
114 | 7 | 16 |
| (R)-(+)-Perillyl alcohol | 106 | 12 | 8.8 | |
| 321-MB | 308 | 40 | 7.7 | |
| 2-Buten-1-ol | 202 | 44 | 4.6 | |
| Cinnamyl alcoholb | 132 | 36 | 3.7 | |
| 3-Methyl-1-butanol | 57 | 270 | 0.2 | |
| 3-Methyl-3-buten-1-ol | 128 | 1,700 | 0.08 | |
| 1-Butanol | 53 | 1,100 | 0.05 | |
| Aromatic 3-Aminobenzyl alcohol | 418 | 11 | 38 | |
| Benzyl alcohol | 155 | 7 | 22 | |
a
Values were determined as described in Materials and Methods.
b
Cinnamyl alcohol, which contains an aromatic ring, is listed with the allylic substrates because it contains an allylic alcohol moiety.