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. 2022 Apr 23;12(5):268. doi: 10.3390/bios12050268

Figure 1.

Figure 1

Cas14a1 Fluorescence Detection System. (a) Schematic of Cas14a1 fluorescence detection system. (b) Identification of Cas14a1 protein after purification: left, identification of Cas14a1 by Western blotting; right, Cas14a1 purity assessed using SDS-PAGE; BSA: control (lane 1). (c) Comparison of fluorescence signals obtained using different buffer candidates; ****p < 0.0001. (d) Comparison of fluorescence signals obtained using different lengths of FQ probe: FQ-12nt, length of FQ probe = 12 nt; FQ-21nt, length of FQ probe = 21 nt.