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. 2022 May 11;11(5):733. doi: 10.3390/biology11050733

Table 1.

Conditioning methods.

Type of Conditioning Conditioning Method Reference
Chemical induction Standard osteogenic medium [29,31,33,36,40]
Dimethyloxalylglycine [20]
Dexamethasone [30]
Strontium-substituted calcium silicate ceramics [41]
Chemical induction + culture conditions Standart osteogenic medium + serum deprivation [32,34,35,37]
Genetic modification miR-375-overexpressing ASCs [38]
Genetic modification + culture conditions BMP2-overexpressing BMSCs + serum deprivation [21]
Mutant HIF-1α-modified BMSCs + serum deprivation [18,28]
Culture conditions Hypoxia [17]
Three-dimensional mechanical microenvironment [39]

ASCs, adipose-tissue-derived stem cells; BMP2, bone morphogenetic protein 2; BMSCs, bone marrow mesenchymal stem cells; HIF, hypoxia-inducible factor.