FIG. 6.

Synthesis of CebE and MsiK proteins. Wild-type S. reticuli was grown in minimal medium with the following saccharide(s) at 0.5% (wt/vol) each: glucose (lane 1), cellobiose (lane 2), cellobiose plus glucose (lane 3), cellobiose plus maltose (lane 4), cellobiose plus sucrose (lane 5), maltose (lane 6), and sucrose (lane 7). Mycelia were disrupted by sonification, and 20 μg of protein per lane was separated by SDS-PAGE (17). Immunodetection of CebE (A) and MsiK (B) proteins was performed as outlined previously (35).