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Global O-GlcNAcylation is decreased in XEN compared to ES cells: (a) Western blot, immunodot blot, and immunodot blot densitometry of O-GlcNAcylated proteins from ESC and XEN cells detected by the RL2 antibody; (b) expression levels and ratio of Ogt and Oga transcripts and (c) expression levels of Gfpt1 and Gfpt2 in ES and XEN cells as detected by RT-qPCR. Bars represent mean ± SEM, N = 3. * p < 0.05, ** p < 0.01.
