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. 2022 Apr 22;12(5):623. doi: 10.3390/biom12050623

Figure 2.

Figure 2

OGA inhibition alters pluripotency marker expression in ES cells and XEN marker expression in XEN cells. The cells were treated with 10 μM TG for 24 h: (a) immunodot blot and densitometry of O-GlcNAcylated proteins detected by the RL2 antibody in XEN cells; (b,c) expression of pluripotency gene expression Oct4 and Nanog and XEN marker expression FoxA2, Gata6, and Dab2 in ES cells (left panel) and XEN cells (right panel). Bars represent mean values ± SEM, N = 3. * p < 0.05, ** p < 0.01, **** p < 0.0001.