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OGT inhibition in ES cells increases extracellular galectin-3. (a) Immunodot blot of O-GlcNAc detecting antibody RL2 in ES cells treated with 5 mM alloxan, an OGT inhibitor, and densitometry; (b) ELISA measuring secretion of galectin-3 from ES and XEN cells treated with 10 μM TG or 5 mM alloxan for 8 days normalized to total intracellular protein (mg/mL). Bars represent mean values ± SEM, N = 3. * p < 0.05.
