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SDS-PAGE of recombinant Cti-His tag proteins isolated with Ni affinity chromatography under nondenaturating conditions from E. coli M15(pREP4) transformants. (A) Heme peroxidase activity staining with TMBZ. (B) Immunological detection of His-tagged proteins. Lane 1, native Cti; lane 2, Cti with amino acid exchange C46S.
