TABLE 2.
Effect of ethanol on cis-trans isomerization of unsaturated fatty acids in different strainsa
| Strain | Ethanol addition | Fatty acid content (%)b
|
|||||
|---|---|---|---|---|---|---|---|
| 16:0 | 16:1 cis | 16:1 trans | 18:0 | 18:1 cis | 18:1 trans | ||
| P. putida P8 | − | 33.9 ± 1.7 | 33.4 ± 4.6 | 8.1 ± 3.8 | 2.1 ± 0.3 | 20.0 ± 2.2 | 2.3 ± 1.7 |
| + | 33.4 ± 1.0 | 21.2 ± 2.4 | 18.8 ± 4.5 | 2.9 ± 1.7 | 17.9 ± 2.1 | 3.5 ± 2.2 | |
| P. putida A9 | − | 32.5 ± 0.8 | 44.8 ± 1.2 | 0 | 1.7 ± 0.3 | 20.9 ± 0.3 | 0 |
| + | 32.4 ± 1.8 | 43.6 ± 1.7 | 0 | 1.8 ± 0.5 | 21.5 ± 0.7 | 0 | |
| P. putida S12 | − | 34.0 ± 2.0 | 33.3 ± 1.5 | 1.4 ± 1.6 | 2.7 ± 0.4 | 27.4 ± 2.0 | 1.1 ± 0.7 |
| + | 33.9 ± 1.6 | 29.9 ± 2.0 | 5.0 ± 1.7 | 2.5 ± 0.3 | 27.0 ± 1.9 | 1.5 ± 0.5 | |
a
Strains were grown in minimal medium to an optical density at 600 nm of 0.3 at 30°C before ethanol was added to a final concentration of 10%. Fatty acid determination was done 2 h thereafter in ethanol-treated (+) and control (−) cells.
b
Fatty acids were assayed as described in Materials and Methods; values for only the relevant fatty acids are given. The values are the means from at least quadruplicate experiments; standard deviations are also indicated.