Association of pre-operative levels of sLAG3 and relapse in lung cancer. (A) Receiver-operating-characteristic (ROC) curve describing the predictive performance value of plasma sLAG3 for relapse in patients with lung cancer (n = 83) (black line; area under the curve [AUC], 0.867 [95% CI 0.770–0.965]) is shown. Optimal cut-off, estimated by the Youden index for plasma sLAG3 concentration, 722.5 pg/mL (red point shows optimal Youden cut-off specificity and sensitivity values). The ROC curve analysis was performed by Wilson/Brown test, with 95% confidence interval. (B) Chart shows number of patients who suffer relapse in the sLAG3-High group (>722.5 ng/mL) compared with the sLAG3-Low group (<722.5 ng/mL). Differences between groups were analysed with the chi-squared test, ** p = 0.0016. (C) Kaplan–Meier curve from surgery date to relapse or end of study date, according to plasma sLAG3 is shown. The differences between relapse free survival rates associated with sSIGLEC5 were calculated by a log-rank (Mantel-Cox; *** p = 0.0003) test and Gehan–Breslow–Wilcoxon (*** p = 0.0005) test with 95% confidence interval.