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. 2022 May 18;10(5):1160. doi: 10.3390/biomedicines10051160

Figure 6.

Figure 6

Effect of hypercapnia on the expression of mucins in human corneoscleral rim explant cultures. Graphs indicate mean relative mRNA expression of MUC5AC (a) and MUC16 (b) in matched corneoscleral rims following exposure to either 5% CO2 (normocapnia—Nor. Cap) or 20% CO2 (hypercapnia—Hyp. Cap), in vitro for a period of 24 h. The expression of MUC5AC and MUC16 was normalised to expression of β-actin (housekeeping gene). The bar graph indicates mean ± SEM from two technical replicates for each of the three biological replicate experiments. ** p < 0.01; two-tailed paired sample t-test.