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. 2022 May 6;11(5):711. doi: 10.3390/biology11050711

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of RNA-Seq data. Verification of the expression level of the selected eight DEGs from RNA−Seq data through RT-qPCR: (a) Cluster_14363.17023, (b) Cluster_14363.17552, (c) Cluster_14363.18534, (d) Cluster_14363.18706, (e) Cluster_14363.18394, (f) Cluster_14363.19711. Error bars indicate the standard error as mean + SD. The x axis represents the relative expression level, and the y-axis represents cultivars. (g) Correlation coefficient of gene expression between qRT−PCR analysis and RNA-Seq data.