Table 4.
CLL MRD comparison between expert-gated (ground truth) and DNN-gated from serially diluted samples (n = 9 unique cases). The absolute error (delta), for cases with CLL MRD events < 1000 and the proportionate error (% error), for cases with CLL MRD events ≥ 1000, for the total abnormal clonal cell population inference per unknown sample versus the “ground truth” population was determined using the proposed workflow described in Figure 3. For the CLL MRD % WBCs, the absolute difference is shown.
| Case | Dilution | Expert CLL MRD # Events | DNN CLL MRD # Events | Absolute or Proportionate Error for # Events | Expert CLL MRD % WBCs | DNN CLL MRD % WBCs |
Absolute Error for % WBCs |
|---|---|---|---|---|---|---|---|
| CLL-1 | UD | 1,132,442 | 1,096,836 | −3.144% | 88.878 | 88.521 | −0.357 |
| 0.02% | 436 | 431 | −5 | 0.022 | 0.023 | 0.001 | |
| 0.002% | 61 | 60 | −1 | 0.003 | 0.003 | 0.000 | |
| 0.001% | 22 | 24 | 2 | 0.001 | 0.002 | 0.000 | |
| CLL-2 | UD | 1,303,375 | 1,116,751 | −14.319% | 92.812 | 91.454 | −1.357 |
| 0.02% | 492 | 11 | −481 | 0.024 | 0.001 | −0.023 | |
| 0.002% | 29 | 7 | −22 | 0.002 | 0.000 | −0.001 | |
| 0.001% | 17 | 6 | −11 | 0.009 | 0.000 | −0.009 | |
| CLL-3 | UD | 725,221 | 715,315 | −1.366% | 97.025 | 96.949 | −0.075 |
| 0.02% | 465 | 436 | −29 | 0.034 | 0.032 | −0.002 | |
| 0.002% | 62 | 64 | 2 | 0.003 | 0.003 | 0.000 | |
| 0.001% | 22 | 22 | 0 | 0.001 | 0.001 | 0.000 | |
| CLL-4 | UD | 627,879 | 622,034 | −0.931% | 51.493 | 52.536 | 1.042 |
| 0.02% | 327 | 322 | −5 | 0.027 | 0.027 | 0.000 | |
| 0.002% | 33 | 37 | 4 | 0.003 | 0.003 | 0.000 | |
| 0.002% | 38 | 49 | 11 | 0.003 | 0.004 | 0.001 | |
| 0.002% | 29 | 31 | 2 | 0.003 | 0.003 | 0.000 | |
| 0.001% | 11 | 18 | 7 | 0.001 | 0.002 | 0.001 | |
| 0.001% | 12 | 22 | 10 | 0.001 | 0.002 | 0.001 | |
| 0.001% | 20 | 24 | 4 | 0.002 | 0.002 | 0.000 | |
| CLL-5 | UD | 558,396 | 555,029 | −0.603% | 51.997 | 52.340 | 0.343 |
| 0.02% | 368 | 1207 | 227.989% | 0.029 | 0.096 | 0.067 | |
| 0.002% | 28 | 73 | 45 | 0.002 | 0.005 | 0.003 | |
| CLL-6 | UD | 145,914 | 142,952 | −2.03% | 57.279 | 54.866 | −2.413 |
| 0.02% | 324 | 128 | −196 | 0.022 | 0.009 | −0.013 | |
| 0.002% | 45 | 16 | −29 | 0.003 | 0.001 | −0.002 | |
| CLL-7 | UD | 722,712 | 703,261 | −2.691% | 90.674 | 90.722 | 0.048 |
| 0.002% | 70 | 88 | 18 | 0.007 | 0.009 | 0.002 | |
| 0.001% | 49 | 76 | 27 | 0.004 | 0.007 | 0.003 | |
| CLL-8 | UD | 1,139,331 | 1,067,634 | −6.293% | 96.586 | 96.289 | −0.297 |
| 0.02% | 434 | 408 | −26 | 0.025 | 0.023 | −0.002 | |
| 0.002% | 37 | 33 | −4 | 0.002 | 0.002 | 0.000 | |
| CLL-9 | UD | 1,114,004 | 1,086,919 | −2.431% | 96.276 | 96.040 | −0.236 |
| 0.02% | 451 | 358 | −93 | 0.026 | 0.020 | −0.005 | |
| 0.002% | 48 | 41 | −7 | 0.003 | 0.022 | 0.019 |