Figure 1.

Strategy for generation of a conditional knockout of Hif1a. Mice were used which carry loxP sites flanking exon 2 (E2) of Hif1a [26]. Recombination was accomplished by tamoxifen induced expression of Cre from a ROSA26_CreERT2 locus. Loss of exon 2 resulted in the inability of HIF-1α to bind to DNA and HIF-1β. PCR genotyping demonstrated a shortened amplification product in the Hif1a^fl/fl Cre-ER^ki/wt(Cre(ki)) mice after Tam treatment compared to the Hif1a^fl/fl Cre-ER^wt/wt (Cre(-)) mice, indicating the excision of E2. Yellow highlight: Nucleotide sequence of exon 2 of HIF-1α (GenBank AH006789.2).