Figure 9.

Formation of γH2AX foci after IR in HIF-1α knockout mHDC under normoxia or moderate hypoxia, and after treatment with the hydroxylase inhibitor DMOG. The mHDC were incubated in normoxia (NX) or moderate hypoxia (HX), respectively, for 48 h and then irradiated with 5 Gy. The cell cultures were then transferred back to hypoxia or normoxia and stained after the time indicated. Images were taken with a Zeiss LSM510 confocal microscope (A). Fields of vision with more than 50 nuclei were evaluated by counting the γH2AX foci in normoxia (B) or hypoxia (C). The first pairs of bars designated WT23 represent mHDC which contain HIF-1α and were not irradiated. Each experiment was performed three times. Graphs show mean ± SD, groups were compared using two-way ANOVA and the Bonferroni post hoc test, *** p < 0.001.