FIG. 3.

Specificity of the PCR for biotypes 2 and 4 of T. harzianum from cultivated mushrooms. Shown are the amplification products resolved by agarose gel electrophoresis that were generated with primer pair Th-F and Th-R by using genomic DNA templates of eight isolates of T. harzianum biotypes 1, 2, and 4 and T. atroviride (a). Numbers above the lanes refer to PSU accession numbers for the isolates. The arrowheads indicate the position of the predicted 444-bp amplicon. M, 100-bp DNA ladder. N, negative control in which sterile water was substituted for the DNA template.