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. 2022 May 19;11(10):1695. doi: 10.3390/cells11101695

Figure 1.

Figure 1

Hypoxia inhibits mTOR phosphorylation and induces autophagy in DCs. (A) HIF-1α protein levels by Western blotting and BNIP3 mRNA by RT-qPCR analysis, after 24 h exposure to normoxia and hypoxia. (B) phmTOR, mTOR, phULK1, ULK1 and SQSTM1/p62 protein levels by Western blotting. (C) Detection of acidic/lysosomal compartments by Lysotracker and confocal analysis (Scale bar: 15 µm). All blots shown are representative of at least three independent experiments and β-actin was used as loading control and housekeeping gene for RT-qPCR analysis. Asterisk indicates statistically significant differences (p ≤ 0.05; n = 3).