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. 2022 May 19;11(10):1695. doi: 10.3390/cells11101695

Figure 6.

Figure 6

Inhibition of class III PI3K/Vps34 abolishes autophagy in hypoxic LPS-treated DCs. (A) SQSTM1/p62 protein levels as determined by Western blotting in DCs stimulated with LPS, exposed to hypoxia for 24 h and treated or untreated in the last 6 h with SAR405 (5 µM, 10 µM, 20 µM). (B) Detection of acidic/lysosomal compartments by Lysotracker and LAMP1 confocal analysis (scale bar: 15 µm) in LPS stimulated DCs, exposed to hypoxia for 24 h and treated or untreated in the last 6 h with SAR405; LAMP1 protein levels as determined by Western blotting in LPS stimulated DCs, exposed to hypoxia for 48 h and treated or untreated with SAR405. (C) Beclin-1 and Atg5 protein levels as determined by Western blotting in LPS stimulated DCs, exposed to hypoxia for 24 h and 48 h, respectively and treated or untreated in the last 6 h with SAR405. All blots shown are representative of at least three independent experiments and β-actin was used as loading control. * indicates statistically significant differences (p ≤ 0.05; n = 3).