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. 2022 May 19;11(10):1695. doi: 10.3390/cells11101695

Figure 7.

Figure 7

SAR405 affects survival and inflammatory cytokine expression in hypoxic LPS-treated DCs. (A) phAKT, phERK and Mcl-1 protein levels as determined by Western blotting in LPS stimulated DCs, exposed to hypoxia 24 h and treated or untreated in the last 6 h with SAR405. (B) Cell viability (48 h), and IL-6, IL-12 and TNFα mRNAs (24 h), as determined by fluorescein diacetate assay and RT-qPCR analysis, respectively, in LPS stimulated DCs, exposed to hypoxia and treated or untreated in the last 6 h with SAR405. All blots shown are representative of at least three independent experiments and β-actin was used as loading control. β-actin was used as a housekeeping gene for RT-qPCR analysis. * indicates statistically significant differences (p ≤ 0.05; n = 3).