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. 2022 May 12;11(10):1629. doi: 10.3390/cells11101629

Figure 4.

Figure 4

Gene expression in human adipose-derived stem cells (hASCs) after a 24 h cytochalasin B (CB) treatment and after 24 h of recovery time. hASC expression of MKI67, CCND1, CDKN1A, and CDKN2A genes was assessed after 24 h of treatment with CB 1 μM or without treatment (CTR) and 24 h after the chemical’s removal. Data were normalized using four reference genes (GAPDH, RPL13, TBP, and HPRT1); the normalized expression value of untreated cells (CTR) after a 24 h CB treatment was set to 1, and all other gene expression values were reported to CTR value. Graphic represents the normalized fold change ± standard error of mean (SEM); n = 3. Green and orange solid columns refer to CTR cells and to cells treated with CB 1 μM for 24 h, respectively; green and orange dotted columns refer to CTR cells and cells treated with CB 1 μM at the end of a 24 h recovery time, respectively. ** p < 0.01 vs. gene expression of CTR cells (green solid columns); § p < 0.05 vs. gene expression of treated cells at the end of a 24 h CB treatment (green dotted columns).