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. 2022 May 12;11(10):1629. doi: 10.3390/cells11101629

Figure 12.

Figure 12

Immunofluorescence of CCAAT/enhancer-binding protein alpha (C/EBP-α) after 3, 6, and 14 days of induced differentiation in the presence of cytochalasin B. C/EBP-α (red signal) staining was performed in hASC cultures after 3, 6, or 14 days of adipogenic induction in the absence or presence of CB (0.1, 1, 5 and 10 μM) or 0.05% dimethyl sulfoxide (DMSO) (CB vehicle). (A) C/EBP-α expression at 3 (solid-colored columns), 6 (textured, colored columns), and 14 (dotted, colored columns) days of adipogenic differentiation. Graph shows the mean percentages of C/EBP-α-positive cells ± standard deviations (SD); n = 3; * p < 0.05 vs. CTR at 3 days. (B) C/EBP-α (red signal) staining was performed in hASC cultures after 3, 6, and 14 days of adipogenic induction. Nuclei were stained with NucBlueTM (DAPI). Acquisitions of images were made with a Nikon inverted microscope Eclipse Ti2-E and a digital sight camera DS-Qi2, through the imaging software NIS-Elements. Scale bars: 50 μm.