Skip to main content
. 2022 May 12;11(10):1629. doi: 10.3390/cells11101629

Figure 14.

Figure 14

Adipogenic potential of human adipose-derived stem cells (hASCs) in the presence of cytochalasin B (CB). hASCs were induced to adipogenesis for a total period of 14 days with the StemPro® Adipogenesis Differentiation medium, supplemented with 0.05% dimethyl sulfoxide (DMSO) (CB vehicle) or CB at 0.1, 1, 5, or 10 μM. Cells cultured only with the StemPro® Adipogenesis Differentiation medium were used as a CTR. The final adipogenic commitment was evaluated by Oil Red O (O.R.O) staining. (A) Undifferentiated unstained and O.R.O-stained cells were counted using ImageJ software. The percentage of O.R.O positive cells was calculated. Graph shows the mean percentages of O.R.O positive cells ± standard deviations (SD); n = 3; * p < 0.05 vs. CTR. (B) Representative O.R.O images acquired under phase contrast illumination with the Leica MC170 HD Imaging System. Cells positive for adipogenesis showed red vacuoles in the cytoplasm. Scale bars: 50 μm. (C) White histograms represent data derived from hASCs cultured in basal medium, and colored histograms represent those from hASCs treated with adipogenic medium. The lipid-rich vacuoles detected by O.R.O dye were extracted, and their absorbance was read at 495 nm with a spectrophotometer. Data are expressed as mean of lipid content at 495 nm absorbance (Abs) ± SD. The * represents the significance of differences between data obtained from hASCs cultured in basal medium and hASCs cultured in adipogenic medium (* p < 0.05, n = 3).