Figure 3.

Effects of 3% E-liquids ± Flavors on Multi-Species (S. gordonii, S. intermedius, S. mitis, and S. oralis) Biofilm Formation. Confocal images of mixed streptococci (1:1:1:1) biofilms grown in 50% BHI with 3% E-liquids ± flavorings. The positive control is the 50% BHI alone. The negative control is the 5% Peroxide in 50% BHI. Biofilms were stained with SYTO 59 (red fluorescence). Each image is a typical representative of eight fields of view. Magnification = 630× Z-stacks were acquired at 1 µm thickness of optical slicing. Images are representative of eight different fields of view.