Figure 6.

(A). Pellet Mass before and after E-liquid or stock flavor treatments. The weight of oral streptococci pellets was determined before and after treatment with 100 µL of PBS, or 100 µL of flavorless E-liquid, or cinnamon or menthol stock flavorings. Each bar represents mean ± SEM (n = 3). ** p < 0.01, *** p < 0.001. (B). Pellet size of representative centrifuge tubes. Bacteria were exposed to 100 µL of PBS, flavorless E-liquid, or cinnamon and menthol flavoring agents for 15 min. Bacteria were then washed and pelleted. Green arrows indicate precipitation of menthol crystals and orange arrows indicate the presence of a hydrophobic material associated with the cinnamon flavor.